| Summary: | BACKGROUND: Diisopropylcarbodiimide is used as a chemical reagent. We tested if diisopropylcarbodiimide could cause cancer in two different strains of genetically modified mice. METHODS: We applied solutions containing diisopropylcarbodiimide in ethanol to the backs of female Tg.AC mice five times per week for 20 weeks and to female p53 haploinsufficient mice for 27 weeks. The daily doses were 4.38, 8.75, 17.5, 35, or 70 milligrams of diisopropylcarbodiimide per kilogram body weight. Animals given the ethanol solution alone served as the control groups. Tissues from over 20 sites were examined for every animal. RESULTS: Exposure to diisopropylcarbodiimide had no effect on the Tg.AC mice. In p53 haploinsufficient mice, the only response seen was an increase in minimal epidermal hyperplasia in the skin at the site of dermal application in mice receiving 70 milligrams of diisopropylcarbodiimide per kilogram of body weight. CONCLUSIONS: We conclude that diisopropylcarbodiimide did not cause cancer in the genetically modified mice used in these studies
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