Vaccine Delivery Technology Methods and Protocols
This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation of viral vacci...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2021, 2021
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| Edition: | 1st ed. 2021 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder
- The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers
- Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice
- The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot
- The Measurement of Antigen-Specific IgG Titers by Direct ELISA
- Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model
- Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils
- Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology
- Vaccine Delivery and Immune Response Basics
- Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes
- Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies
- Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed
- Antigen Discovery in Bacterial Pan-Proteomes
- Alphavirus-Based Antigen Preparation
- Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells
- Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System
- High-Throughput Process Development for the Chromatographic Purification of Viral Antigens
- Production of Zika Virus Virus-Like Particles
- Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs)
- Up- and Downstream Processes for Viral Nanoplaxes as Vaccines
- Whole-Cell Vaccine Preparation: Options and Perspectives
- O-AntigenExtraction, Purification, and Chemical Conjugation to a Carrier Protein
- Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines
- Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines
- Attenuation Methods for Live Vaccines
- Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism
- Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells
- Lipofection-Based Delivery of DNA Vaccines
- Flavivirus DNA Vaccine Design and Adjuvant Selection
- Vaccine Delivery with a Detoxified Bacterial Toxin
- Needle-Less or Non-Invasive Delivery Technology
- Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications
- A Hybrid Biological-Biomaterial Vector for Antigen Delivery
- Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens