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| 008 |
201006 ||| eng |
| 020 |
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|a 9781071607954
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| 100 |
1 |
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|a Pfeifer, Blaine A.
|e [editor]
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| 245 |
0 |
0 |
|a Vaccine Delivery Technology
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Blaine A. Pfeifer, Andrew Hill
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| 250 |
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|a 1st ed. 2021
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| 260 |
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|a New York, NY
|b Humana
|c 2021, 2021
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| 300 |
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|a XV, 590 p. 149 illus., 127 illus. in color
|b online resource
|
| 505 |
0 |
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|a Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder -- The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers -- Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice -- The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot -- The Measurement of Antigen-Specific IgG Titers by Direct ELISA -- Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model -- Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils -- Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology
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| 505 |
0 |
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|a Vaccine Delivery and Immune Response Basics -- Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes -- Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies -- Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed -- Antigen Discovery in Bacterial Pan-Proteomes -- Alphavirus-Based Antigen Preparation -- Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells -- Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System -- High-Throughput Process Development for the Chromatographic Purification of Viral Antigens -- Production of Zika Virus Virus-Like Particles -- Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs) -- Up- and Downstream Processes for Viral Nanoplaxes as Vaccines -- Whole-Cell Vaccine Preparation: Options and Perspectives --
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| 505 |
0 |
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|a O-AntigenExtraction, Purification, and Chemical Conjugation to a Carrier Protein -- Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines -- Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines -- Attenuation Methods for Live Vaccines -- Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism -- Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells -- Lipofection-Based Delivery of DNA Vaccines -- Flavivirus DNA Vaccine Design and Adjuvant Selection -- Vaccine Delivery with a Detoxified Bacterial Toxin -- Needle-Less or Non-Invasive Delivery Technology -- Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications -- A Hybrid Biological-Biomaterial Vector for Antigen Delivery -- Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens --
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| 653 |
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|a Biological Techniques
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| 653 |
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|a Chemistry
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| 653 |
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|a Immunology
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| 653 |
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|a Biotechnology
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| 653 |
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|a Pharmacology
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| 653 |
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|a Biology / Technique
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| 653 |
|
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|a Biomaterials
|
| 700 |
1 |
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|a Hill, Andrew
|e [editor]
|
| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
|
| 989 |
|
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|b Springer
|a Springer eBooks 2005-
|
| 490 |
0 |
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|a Methods in Molecular Biology
|
| 028 |
5 |
0 |
|a 10.1007/978-1-0716-0795-4
|
| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-0716-0795-4?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
0 |
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|a 540
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| 520 |
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|a This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation of viral vaccines as VLPs; viral and non-viral gene delivery; needle-less or non-invasive delivery technology; vaccine storage; and vaccine administration and assessment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vaccine Delivery Technology: Methods and Protocols is a valuable resource for both novice and expert researchers, in and outside the field, who would like to gain insight into the impactful field of vaccines. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com
|