In Vitro Mutagenesis Protocols
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
1996, 1996
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| Edition: | 1st ed. 1996 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR)
- Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides
- Random Sequence Mutagenesis for the Generation of Active Enzymes
- Random Mutagenesis by Using Mixtures of dNTP and dITP in PCR
- PCR-Mediated Chemical Mutagenesis
- Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method
- An Efficient Random Mutagenesis Technique Using an E. coli Mutator Strain
- In Vitro Recombination and Mutagenesis by Overlap Extension PCR
- Site-Directed Mutagenesis Using Overlap Extension PCR
- Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template
- Site-Directed Mutagenesis In Vitro by Megaprimer PCR
- Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids
- PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments
- Site-Directed Mutagenesis Using a Rapid PCR-Based Method
- A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Sequence
- A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered
- Multiple Site-Directed Mutagenesis
- Construction of Linker-Scanning Mutations by Oligonucleotide Ligation
- Construction of Linker-Scanning Mutations Using PCR
- Use of Codon Cassette Mutagenesis for Saturation Mutagenesis
- Site-Directed Mutagenesis Using Positive Antibiotic Selection
- In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method
- Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates
- Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template
- Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach
- Analysis of Point Mutations by Use of Amber Stop Codon Suppression
- A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA
- Double-Stranded DNA Site-Directed Mutagenesis
- Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template
- Targeted Mutagenesis Mediated by the Triple Helix Formation
- A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site
- Ordered Deletions Using Exonuclease III
- Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis
- PCR-Based Site-Directed Mutagenesis