In Vitro Mutagenesis Protocols

Bibliographic Details
Other Authors: Trower, Michael K. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 1996, 1996
Edition:1st ed. 1996
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
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245 0 0 |a In Vitro Mutagenesis Protocols  |h Elektronische Ressource  |c edited by Michael K. Trower 
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300 |a XIV, 391 p  |b online resource 
505 0 |a Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR) -- Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides -- Random Sequence Mutagenesis for the Generation of Active Enzymes -- Random Mutagenesis by Using Mixtures of dNTP and dITP in PCR -- PCR-Mediated Chemical Mutagenesis -- Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method -- An Efficient Random Mutagenesis Technique Using an E. coli Mutator Strain 
505 0 |a In Vitro Recombination and Mutagenesis by Overlap Extension PCR -- Site-Directed Mutagenesis Using Overlap Extension PCR -- Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template -- Site-Directed Mutagenesis In Vitro by Megaprimer PCR -- Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids -- PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments -- Site-Directed Mutagenesis Using a Rapid PCR-Based Method -- A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Sequence -- A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered -- Multiple Site-Directed Mutagenesis -- Construction of Linker-Scanning Mutations by Oligonucleotide Ligation -- Construction of Linker-Scanning Mutations Using PCR -- Use of Codon Cassette Mutagenesis for Saturation Mutagenesis --  
505 0 |a Site-Directed Mutagenesis Using Positive Antibiotic Selection -- In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method -- Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates -- Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template -- Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach -- Analysis of Point Mutations by Use of Amber Stop Codon Suppression -- A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA -- Double-Stranded DNA Site-Directed Mutagenesis -- Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template -- Targeted Mutagenesis Mediated by the Triple Helix Formation -- A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site -- Ordered Deletions Using Exonuclease III -- Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis -- PCR-Based Site-Directed Mutagenesis --  
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