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191115 ||| eng |
020 |
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|a 9781592595440
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100 |
1 |
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|a Trower, Michael K.
|e [editor]
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245 |
0 |
0 |
|a In Vitro Mutagenesis Protocols
|h Elektronische Ressource
|c edited by Michael K. Trower
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250 |
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|a 1st ed. 1996
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260 |
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|a Totowa, NJ
|b Humana
|c 1996, 1996
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300 |
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|a XIV, 391 p
|b online resource
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505 |
0 |
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|a Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR) -- Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides -- Random Sequence Mutagenesis for the Generation of Active Enzymes -- Random Mutagenesis by Using Mixtures of dNTP and dITP in PCR -- PCR-Mediated Chemical Mutagenesis -- Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method -- An Efficient Random Mutagenesis Technique Using an E. coli Mutator Strain
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505 |
0 |
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|a In Vitro Recombination and Mutagenesis by Overlap Extension PCR -- Site-Directed Mutagenesis Using Overlap Extension PCR -- Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template -- Site-Directed Mutagenesis In Vitro by Megaprimer PCR -- Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids -- PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments -- Site-Directed Mutagenesis Using a Rapid PCR-Based Method -- A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Sequence -- A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered -- Multiple Site-Directed Mutagenesis -- Construction of Linker-Scanning Mutations by Oligonucleotide Ligation -- Construction of Linker-Scanning Mutations Using PCR -- Use of Codon Cassette Mutagenesis for Saturation Mutagenesis --
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505 |
0 |
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|a Site-Directed Mutagenesis Using Positive Antibiotic Selection -- In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method -- Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates -- Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template -- Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach -- Analysis of Point Mutations by Use of Amber Stop Codon Suppression -- A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA -- Double-Stranded DNA Site-Directed Mutagenesis -- Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template -- Targeted Mutagenesis Mediated by the Triple Helix Formation -- A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site -- Ordered Deletions Using Exonuclease III -- Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis -- PCR-Based Site-Directed Mutagenesis --
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653 |
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|a Cell Biology
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653 |
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|a Cytology
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041 |
0 |
7 |
|a eng
|2 ISO 639-2
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989 |
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|b SBA
|a Springer Book Archives -2004
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490 |
0 |
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|a Methods in Molecular Biology
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028 |
5 |
0 |
|a 10.1385/0896033325
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856 |
4 |
0 |
|u https://doi.org/10.1385/0896033325?nosfx=y
|x Verlag
|3 Volltext
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082 |
0 |
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|a 571.6
|