Receptor Signal Transduction Protocols
In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combin...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2004, 2004
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| Edition: | 2nd ed. 2004 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Radioligand-Binding Methods for Membrane Preparations and Intact Cells
- Determination of Allosteric Interactions Using Radioligand-Binding Techniques
- Generation, Use, and Validation of Receptor-Selective Antibodies
- Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization
- Generation and Use of Epitope-Tagged Receptors
- Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and In Situ Hybridization
- Electron Microscopic Studies of Receptor Localization
- Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors
- Viral Infection Protocols
- Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection
- Covalent Modification of G-Proteins by Affinity Labeling
- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Assess Total G-Protein and G?-Subtype-Specific Activation by G-Protein-Coupled Receptors
- Identification and Quantitation of G-Protein ?-Subunits
- Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins
- Assessment of Receptor Internalization and Recycling
- G-Protein-Coupled Receptor Phosphorylation and Palmitoylation
- Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping
- Ubiquitination of G-Protein-Coupled Receptors
- Receptor Mutagenesis Strategies for Examination of Structure-Function Relationships
- Study of G-Protein-Coupled Receptor-Protein Interactions by Bioluminescence Resonance Energy Transfer
- Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cells
- Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitation
- Study of G-Protein-Coupled Receptor-Protein Interactions Using Gel Overlay Assaysand Glutathione-S-Transferase-Fusion Protein Pull-Downs
- Receptor Knock-Out and Knock-In Strategies
- Statistical Methods in G-Protein-Coupled Receptor Research