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191115 ||| eng |
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|a 9781592597543
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| 100 |
1 |
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|a Willars, Gary B.
|e [editor]
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| 245 |
0 |
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|a Receptor Signal Transduction Protocols
|h Elektronische Ressource
|c edited by Gary B. Willars, R. A. John Challiss
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| 250 |
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|a 2nd ed. 2004
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| 260 |
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|a Totowa, NJ
|b Humana
|c 2004, 2004
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| 300 |
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|a XIII, 418 p
|b online resource
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| 505 |
0 |
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|a Radioligand-Binding Methods for Membrane Preparations and Intact Cells -- Determination of Allosteric Interactions Using Radioligand-Binding Techniques -- Generation, Use, and Validation of Receptor-Selective Antibodies -- Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization -- Generation and Use of Epitope-Tagged Receptors -- Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and In Situ Hybridization -- Electron Microscopic Studies of Receptor Localization -- Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors -- Viral Infection Protocols -- Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection -- Covalent Modification of G-Proteins by Affinity Labeling -- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Assess Total G-Protein and G?-Subtype-Specific Activation by G-Protein-Coupled Receptors -- Identification and Quantitation of G-Protein ?-Subunits -- Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins -- Assessment of Receptor Internalization and Recycling -- G-Protein-Coupled Receptor Phosphorylation and Palmitoylation -- Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping -- Ubiquitination of G-Protein-Coupled Receptors -- Receptor Mutagenesis Strategies for Examination of Structure-Function Relationships -- Study of G-Protein-Coupled Receptor-Protein Interactions by Bioluminescence Resonance Energy Transfer -- Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cells -- Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitation -- Study of G-Protein-Coupled Receptor-Protein Interactions Using Gel Overlay Assaysand Glutathione-S-Transferase-Fusion Protein Pull-Downs -- Receptor Knock-Out and Knock-In Strategies -- Statistical Methods in G-Protein-Coupled Receptor Research
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| 653 |
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|a Cell Biology
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| 653 |
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|a Cytology
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| 700 |
1 |
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|a Challiss, R. A. John
|e [editor]
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 490 |
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|a Methods in Molecular Biology
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| 028 |
5 |
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|a 10.1385/1592597548
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| 856 |
4 |
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|u https://doi.org/10.1385/1592597548?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 571.6
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| 520 |
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|a In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combines updates of key chapters from the first edition with a large number of new contributions on the many successful methodologies that have emerged more recently. The methods are focused primarily on events at the receptor level, including ligand binding, on the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR-protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Receptor Signal Transduction Protocols, Second Edition offers both novice and experienced investigators powerful cutting-edge techniques that provide an array of approaches and specific methods that will aid in the understanding of GPCR structure and function
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