Plant Pattern Recognition Receptors Methods and Protocols

This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written...

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Bibliographic Details
Other Authors: Shan, Libo (Editor), He, Ping (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2017, 2017
Edition:1st ed. 2017
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Peptidoglycan Isolation and Binding Studies with LysM-Type Pattern Recognition Receptors
  • Characterization of Plant Cell Wall Damage-associated Molecular Patterns Regulating Immune Responses
  • Methods of Isolation and Characterization of Oligogalacturonide Elicitors
  • Quantitative Analysis of Ligand-induced Endocytosis of FLAGELLIN-SENSING 2 using Automated Image Segmentation
  • Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants
  • Assays to Investigate the N-glycosylation State and Function of Plant Pattern Recognition Receptors
  • Steady-State and Kinetics-Based Affinity Determination in Effector-Effector Target Interactions
  • In Vitro Ubiquitination Activity Assays in Plant Immune Responses
  • Bioinformatics Analysis of the Receptor-Like Kinase (RLK) Superfamily
  • Identification of MAPK Substrates using Quantitative Phosphoproteomics
  • Analysis of PAMP-Triggered ROS Burst in Plant Immunity
  • Small RNA and mRNA Profiling of Arabidopsis Infected by Phytophthora
  • Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants
  • Rapid Construction of Multiplexed CRISPR-Cas9 Systems for Plant Genome Editing
  • Chitin-triggered MAPK activation and ROS generation in Rice Suspension-cultured Cells
  • Chitin Induced Responses in the Moss Physcomitrella patens
  • Methods to Quantify PAMP-Triggered MAP Kinase Phosphorylation, Oxidative Burst, and Lignification in Brassicas
  • Effectoromics-Based Identification of Cell Surface Receptors in Potato.
  • LeEIX2 interactors’ Analysis and EIX-mediated Response Measurement
  • MAPK Assays in MAMP-PRR Signal Transduction Pathway in Arabidopsis
  • CDPK Activation in PRR Signaling
  • Protein Kinase Assays
  • Measuring Callose Deposition, an Indicator of Cell Wall Reinforcement, during Bacterial Infection in Arabidopsis
  • Quantitative Evaluation of Plant Actin Cytoskeletal Organization during Immune Signaling
  • Network Reconstitution for Quantitative Subnetwork Interaction Analysis
  • Stomatal Bioassay to Characterize Bacterial-stimulated PTI at the Pre-invasion Phase of Infection
  • Using Clear Nail Polish to Make Arabidopsis Epidermal Impressions for Measuring the Change of Stomatal Aperture Size in Immune Response
  • Characterizing the Immune-Eliciting Activity of Putative Microbe-Associated Molecular Patterns in Tomato
  • Genome-Wide Analysis of Chromatin Accessibility in Arabidopsis Infected with Pseudomonas syringae