Plant Pattern Recognition Receptors Methods and Protocols

This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written...

Full description

Bibliographic Details
Other Authors: Shan, Libo (Editor), He, Ping (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2017, 2017
Edition:1st ed. 2017
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
LEADER 04171nmm a2200301 u 4500
001 EB001346282
003 EBX01000000000000000900472
005 00000000000000.0
007 cr|||||||||||||||||||||
008 170301 ||| eng
020 |a 9781493968596 
100 1 |a Shan, Libo  |e [editor] 
245 0 0 |a Plant Pattern Recognition Receptors  |h Elektronische Ressource  |b Methods and Protocols  |c edited by Libo Shan, Ping He 
250 |a 1st ed. 2017 
260 |a New York, NY  |b Springer New York  |c 2017, 2017 
300 |a XIV, 358 p. 57 illus., 39 illus. in color  |b online resource 
505 0 |a Peptidoglycan Isolation and Binding Studies with LysM-Type Pattern Recognition Receptors -- Characterization of Plant Cell Wall Damage-associated Molecular Patterns Regulating Immune Responses -- Methods of Isolation and Characterization of Oligogalacturonide Elicitors -- Quantitative Analysis of Ligand-induced Endocytosis of FLAGELLIN-SENSING 2 using Automated Image Segmentation -- Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants -- Assays to Investigate the N-glycosylation State and Function of Plant Pattern Recognition Receptors -- Steady-State and Kinetics-Based Affinity Determination in Effector-Effector Target Interactions -- In Vitro Ubiquitination Activity Assays in Plant Immune Responses -- Bioinformatics Analysis of the Receptor-Like Kinase (RLK) Superfamily -- Identification of MAPK Substrates using Quantitative Phosphoproteomics -- Analysis of PAMP-Triggered ROS Burst in Plant Immunity --  
505 0 |a Small RNA and mRNA Profiling of Arabidopsis Infected by Phytophthora -- Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants -- Rapid Construction of Multiplexed CRISPR-Cas9 Systems for Plant Genome Editing -- Chitin-triggered MAPK activation and ROS generation in Rice Suspension-cultured Cells -- Chitin Induced Responses in the Moss Physcomitrella patens -- Methods to Quantify PAMP-Triggered MAP Kinase Phosphorylation, Oxidative Burst, and Lignification in Brassicas -- Effectoromics-Based Identification of Cell Surface Receptors in Potato. 
505 0 |a LeEIX2 interactors’ Analysis and EIX-mediated Response Measurement -- MAPK Assays in MAMP-PRR Signal Transduction Pathway in Arabidopsis -- CDPK Activation in PRR Signaling -- Protein Kinase Assays -- Measuring Callose Deposition, an Indicator of Cell Wall Reinforcement, during Bacterial Infection in Arabidopsis -- Quantitative Evaluation of Plant Actin Cytoskeletal Organization during Immune Signaling -- Network Reconstitution for Quantitative Subnetwork Interaction Analysis -- Stomatal Bioassay to Characterize Bacterial-stimulated PTI at the Pre-invasion Phase of Infection -- Using Clear Nail Polish to Make Arabidopsis Epidermal Impressions for Measuring the Change of Stomatal Aperture Size in Immune Response -- Characterizing the Immune-Eliciting Activity of Putative Microbe-Associated Molecular Patterns in Tomato -- Genome-Wide Analysis of Chromatin Accessibility in Arabidopsis Infected with Pseudomonas syringae --  
653 |a Botany 
653 |a Plant Science 
700 1 |a He, Ping  |e [editor] 
041 0 7 |a eng  |2 ISO 639-2 
989 |b Springer  |a Springer eBooks 2005- 
490 0 |a Methods in Molecular Biology 
856 4 0 |u https://doi.org/10.1007/978-1-4939-6859-6?nosfx=y  |x Verlag  |3 Volltext 
082 0 |a 580 
520 |a This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocolsaims to ensure successful results in the further study of this vital field