Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
This volume provides current methods and protocols for gene and protein delivery based on both lentivirus-generated and spontaneously released nanovesicles. Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools guides readers through methods on macromolecule delivery and chapters descri...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Springer New York
2016, 2016
|
| Edition: | 1st ed. 2016 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown
- Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase
- Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions
- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G
- Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors
- Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors
- Production and Concentration of Lentivirus for Transduction of Primary Human T Cells
- Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer
- The LAM-PCR Method to Sequence LV Integration Sites
- Conditional Rnai using the Lentiviral GLTR System
- Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies
- Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line
- Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease
- Development of Lentiviral Vectors for Targeted Integration and Protein Delivery
- Biogenesis and Functions of Exosomes and Extracellular Vesicles
- Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes
- Cardiac Myocyte Exosome Isolation
- Incorporation of Heterologous Proteins in Engineered Exosomes
- Exosome-Mediated Targeted Delivery of Mirnas