|
|
|
|
| LEADER |
03411nmm a2200277 u 4500 |
| 001 |
EB001227551 |
| 003 |
EBX01000000000000000870854 |
| 005 |
00000000000000.0 |
| 007 |
cr||||||||||||||||||||| |
| 008 |
160701 ||| eng |
| 020 |
|
|
|a 9781493937530
|
| 100 |
1 |
|
|a Federico, Maurizio
|e [editor]
|
| 245 |
0 |
0 |
|a Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
|h Elektronische Ressource
|c edited by Maurizio Federico
|
| 250 |
|
|
|a 1st ed. 2016
|
| 260 |
|
|
|a New York, NY
|b Springer New York
|c 2016, 2016
|
| 300 |
|
|
|a XI, 276 p. 47 illus., 33 illus. in color
|b online resource
|
| 505 |
0 |
|
|a Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown -- Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase -- Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions -- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G -- Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors -- Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors -- Production and Concentration of Lentivirus for Transduction of Primary Human T Cells -- Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer -- The LAM-PCR Method to Sequence LV Integration Sites -- Conditional Rnai using the Lentiviral GLTR System -- Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies -- Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line -- Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease -- Development of Lentiviral Vectors for Targeted Integration and Protein Delivery -- Biogenesis and Functions of Exosomes and Extracellular Vesicles -- Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes -- Cardiac Myocyte Exosome Isolation -- Incorporation of Heterologous Proteins in Engineered Exosomes -- Exosome-Mediated Targeted Delivery of Mirnas
|
| 653 |
|
|
|a Human Genetics
|
| 653 |
|
|
|a Human genetics
|
| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
|
| 989 |
|
|
|b Springer
|a Springer eBooks 2005-
|
| 490 |
0 |
|
|a Methods in Molecular Biology
|
| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-4939-3753-0?nosfx=y
|x Verlag
|3 Volltext
|
| 082 |
0 |
|
|a 599.935
|
| 082 |
0 |
|
|a 611.01816
|
| 520 |
|
|
|a This volume provides current methods and protocols for gene and protein delivery based on both lentivirus-generated and spontaneously released nanovesicles. Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools guides readers through methods on macromolecule delivery and chapters describe the LV-based protocols of gene engineering. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools aims to ensure successful results in the further study of this vital field
|