Innate DNA and RNA Recognition Methods and Protocols

Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists...

Full description

Bibliographic Details
Other Authors: Anders, Hans-Joachim (Editor), Migliorini, Adriana (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2014, 2014
Edition:1st ed. 2014
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
  • Enzymatic Synthesis and Purification of a Defined RIG-I Ligand
  • Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis
  • Isolation of RIG-I-associated RNAs from Virus Infected Cells
  • Structure Modeling of Toll-like Receptors
  • Nucleic Acid Recognition in Dendritic Cell
  • Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems
  • Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro
  • In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes
  • Mapping of Optimal CD8 T Cell Epitopes
  • A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection
  • MicroRNA Methodology: Advances in miRNA-technologies
  • Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR)
  • Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus
  • Induction and Analysis of Nephrotoxic Serum Nephritis in Mice
  • Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice
  • Bifunctional siRNAs for Tumor Therapy