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|a 9781493908820
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|a Anders, Hans-Joachim
|e [editor]
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|a Innate DNA and RNA Recognition
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Hans-Joachim Anders, Adriana Migliorini
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|a 1st ed. 2014
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|a New York, NY
|b Humana
|c 2014, 2014
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|a XI, 195 p. 37 illus., 16 illus. in color
|b online resource
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|a Detection of RNA Modifications by HPLC Analysis and Competitive ELISA -- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand -- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis -- Isolation of RIG-I-associated RNAs from Virus Infected Cells -- Structure Modeling of Toll-like Receptors -- Nucleic Acid Recognition in Dendritic Cell -- Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems -- Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro -- In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes -- Mapping of Optimal CD8 T Cell Epitopes -- A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection -- MicroRNA Methodology: Advances in miRNA-technologies -- Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) -- Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus -- Induction and Analysis of Nephrotoxic Serum Nephritis in Mice -- Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice -- Bifunctional siRNAs for Tumor Therapy
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|a Medical Genetics
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|a Medical genetics
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|a Migliorini, Adriana
|e [editor]
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|a eng
|2 ISO 639-2
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|b Springer
|a Springer eBooks 2005-
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|a Methods in Molecular Biology
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|a 10.1007/978-1-4939-0882-0
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|u https://doi.org/10.1007/978-1-4939-0882-0?nosfx=y
|x Verlag
|3 Volltext
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|a 616.042
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|a Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists, and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources
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