Protein Structure Analysis Preparation, Characterization, and Microsequencing

The structure of a protein is essential for its function. Therefore, a variety of methods for analyzing protein structure have been developed recently; the most advanced and sensitive techniques have been selected for this manual. The presented procedures are well established and have been found use...

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Bibliographic Details
Other Authors: Kamp, Roza Maria (Editor), Choli-Papadopoulou, Theodora (Editor), Wittmann-Liebold, Brigitte (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg Springer Berlin Heidelberg 1997, 1997
Edition:1st ed. 1997
Series:Springer Lab Manuals
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • I Separation of Proteins
  • 1 Purification of Proteins for Sequencing
  • 2 Affinity Chromatography of Proteins
  • 3 Protein Purification by Aqueous Two-Phase Systems
  • 4 Rapid HPLC Separation of Proteins, Peptides, and Amino Acids Using Short Columns Filled with Nonporous Silica-Based Particles
  • II Separation of Peptides
  • 5 Enzymatic and Chemical Cleavages of Proteins
  • 6 Separation of Peptides Using HPLC and TLC Fingerprints
  • 7 Microseparation of In Situ Digested Peptides
  • 8 Isolation of Peptides for Microsequencing by In-Gel Proteolytic Digestion
  • III Manual and Automated Microsequencing Methods
  • 9 Automated Microsequencing: Introduction and Overview
  • 10 A Manual Method for Protein Sequence Analysis Using the DABITC/PITC Method
  • 11 Solid-Phase Sequencing of Peptides and Proteins
  • 12 Sequence Analysis of the NH2-Terminally Blocked Proteins Immobilized on PVDF Membranes from Polyacrylamide Gels
  • IV Electrophoresis and Blotting of Proteins
  • 13 Two-Dimensional Electrophoresis
  • 14 Semi-Dry Blotting onto Hydrophobic Membranes
  • V Analysis of Amino Acids
  • 15 Highly Sensitive Amino Acid Analysis
  • 16 Quantitative Analysis of D- and L-Amino Acids by HPLC
  • VI Identification of Cysteine Residues and Lipids in Proteins
  • 17 Identification of Cysteine Residues and Disulfide Bonds in Proteins
  • 18 Lipid Modifications of Proteins
  • VII Mass Spectrometry in Peptide and Protein Analysis
  • 19 Mass Spectrometry in Peptide and Protein Sequence Analysis
  • 20 Carboxy-Terminal Sequencing by Combining Carboxypeptidase Y and P Digestion with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
  • 21 Crystallization of Biological Macromolecules