Protein Structure Analysis Preparation, Characterization, and Microsequencing
The structure of a protein is essential for its function. Therefore, a variety of methods for analyzing protein structure have been developed recently; the most advanced and sensitive techniques have been selected for this manual. The presented procedures are well established and have been found use...
Other Authors: | , , |
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Format: | eBook |
Language: | English |
Published: |
Berlin, Heidelberg
Springer Berlin Heidelberg
1997, 1997
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Edition: | 1st ed. 1997 |
Series: | Springer Lab Manuals
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Subjects: | |
Online Access: | |
Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- I Separation of Proteins
- 1 Purification of Proteins for Sequencing
- 2 Affinity Chromatography of Proteins
- 3 Protein Purification by Aqueous Two-Phase Systems
- 4 Rapid HPLC Separation of Proteins, Peptides, and Amino Acids Using Short Columns Filled with Nonporous Silica-Based Particles
- II Separation of Peptides
- 5 Enzymatic and Chemical Cleavages of Proteins
- 6 Separation of Peptides Using HPLC and TLC Fingerprints
- 7 Microseparation of In Situ Digested Peptides
- 8 Isolation of Peptides for Microsequencing by In-Gel Proteolytic Digestion
- III Manual and Automated Microsequencing Methods
- 9 Automated Microsequencing: Introduction and Overview
- 10 A Manual Method for Protein Sequence Analysis Using the DABITC/PITC Method
- 11 Solid-Phase Sequencing of Peptides and Proteins
- 12 Sequence Analysis of the NH2-Terminally Blocked Proteins Immobilized on PVDF Membranes from Polyacrylamide Gels
- IV Electrophoresis and Blotting of Proteins
- 13 Two-Dimensional Electrophoresis
- 14 Semi-Dry Blotting onto Hydrophobic Membranes
- V Analysis of Amino Acids
- 15 Highly Sensitive Amino Acid Analysis
- 16 Quantitative Analysis of D- and L-Amino Acids by HPLC
- VI Identification of Cysteine Residues and Lipids in Proteins
- 17 Identification of Cysteine Residues and Disulfide Bonds in Proteins
- 18 Lipid Modifications of Proteins
- VII Mass Spectrometry in Peptide and Protein Analysis
- 19 Mass Spectrometry in Peptide and Protein Sequence Analysis
- 20 Carboxy-Terminal Sequencing by Combining Carboxypeptidase Y and P Digestion with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
- 21 Crystallization of Biological Macromolecules