Protein Structure Analysis Preparation, Characterization, and Microsequencing

The structure of a protein is essential for its function. Therefore, a variety of methods for analyzing protein structure have been developed recently; the most advanced and sensitive techniques have been selected for this manual. The presented procedures are well established and have been found use...

Full description

Bibliographic Details
Other Authors: Kamp, Roza Maria (Editor), Choli-Papadopoulou, Theodora (Editor), Wittmann-Liebold, Brigitte (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg Springer Berlin Heidelberg 1997, 1997
Edition:1st ed. 1997
Series:Springer Lab Manuals
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
LEADER 03476nmm a2200313 u 4500
001 EB000666826
003 EBX01000000000000000519908
005 00000000000000.0
007 cr|||||||||||||||||||||
008 140122 ||| eng
020 |a 9783642592195 
100 1 |a Kamp, Roza Maria  |e [editor] 
245 0 0 |a Protein Structure Analysis  |h Elektronische Ressource  |b Preparation, Characterization, and Microsequencing  |c edited by Roza Maria Kamp, Theodora Choli-Papadopoulou, Brigitte Wittmann-Liebold 
250 |a 1st ed. 1997 
260 |a Berlin, Heidelberg  |b Springer Berlin Heidelberg  |c 1997, 1997 
300 |a XIII, 316 p  |b online resource 
505 0 |a I Separation of Proteins -- 1 Purification of Proteins for Sequencing -- 2 Affinity Chromatography of Proteins -- 3 Protein Purification by Aqueous Two-Phase Systems -- 4 Rapid HPLC Separation of Proteins, Peptides, and Amino Acids Using Short Columns Filled with Nonporous Silica-Based Particles -- II Separation of Peptides -- 5 Enzymatic and Chemical Cleavages of Proteins -- 6 Separation of Peptides Using HPLC and TLC Fingerprints -- 7 Microseparation of In Situ Digested Peptides -- 8 Isolation of Peptides for Microsequencing by In-Gel Proteolytic Digestion -- III Manual and Automated Microsequencing Methods -- 9 Automated Microsequencing: Introduction and Overview -- 10 A Manual Method for Protein Sequence Analysis Using the DABITC/PITC Method -- 11 Solid-Phase Sequencing of Peptides and Proteins -- 12 Sequence Analysis of the NH2-Terminally Blocked Proteins Immobilized on PVDF Membranes from Polyacrylamide Gels -- IV Electrophoresis and Blotting of Proteins -- 13 Two-Dimensional Electrophoresis -- 14 Semi-Dry Blotting onto Hydrophobic Membranes -- V Analysis of Amino Acids -- 15 Highly Sensitive Amino Acid Analysis -- 16 Quantitative Analysis of D- and L-Amino Acids by HPLC -- VI Identification of Cysteine Residues and Lipids in Proteins -- 17 Identification of Cysteine Residues and Disulfide Bonds in Proteins -- 18 Lipid Modifications of Proteins -- VII Mass Spectrometry in Peptide and Protein Analysis -- 19 Mass Spectrometry in Peptide and Protein Sequence Analysis -- 20 Carboxy-Terminal Sequencing by Combining Carboxypeptidase Y and P Digestion with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry -- 21 Crystallization of Biological Macromolecules 
653 |a Cell Biology 
653 |a Cytology 
653 |a Biochemistry 
700 1 |a Choli-Papadopoulou, Theodora  |e [editor] 
700 1 |a Wittmann-Liebold, Brigitte  |e [editor] 
041 0 7 |a eng  |2 ISO 639-2 
989 |b SBA  |a Springer Book Archives -2004 
490 0 |a Springer Lab Manuals 
028 5 0 |a 10.1007/978-3-642-59219-5 
856 4 0 |u https://doi.org/10.1007/978-3-642-59219-5?nosfx=y  |x Verlag  |3 Volltext 
082 0 |a 572 
520 |a The structure of a protein is essential for its function. Therefore, a variety of methods for analyzing protein structure have been developed recently; the most advanced and sensitive techniques have been selected for this manual. The presented procedures are well established and have been found useful in many laboratories; they are easy to perform and thus also suitable for beginners. Enclosed are protocols on the separation of proteins and peptides, manual and automated microsequencing, electrophoresis and blotting, analysis of amino acids, identification of cysteine residues and lipids as well as on mass spectrometry crystallization of macromolecules