Methods of Protein Separation
This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse quently of...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Springer US
1975, 1975
|
| Edition: | 1st ed. 1975 |
| Series: | Biological Separations
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- 1 Sedimentation and Gel-Permeation Chromatography of Associating-Dissociating Macromolecules: The Role of Ligand Mediation and Rates of Reaction
- I. Introduction
- II. Theoretical Formulation
- III. Results
- IV. Discussion
- V. References
- 2 Trans Electrophoresis
- I. Introduction
- II. Instrumental Aspects
- III. Data Processing
- IV. TRANS-CZE and TRANS-MZE
- V. TRANS-IF
- VI. Future Developments
- VII. References
- 3 Immunodiffusion
- I. Introduction
- II. Antigen-Antibody Reactions
- III. Immunodiffusion
- IV. Immunodiffusion Combined with Electrophoresis
- V. Helpful Hints
- VI. References
- 4 Isoelectric Focusing in Polyacrylamide Gel
- I. Introduction
- II. Background
- III. Methodology
- IV. Sample Detection
- V. Applications
- VI. Discussion
- VII. References
- 5 Purification of Chemically Modified Proteins
- I. Introduction
- II. General Types and Purposes of Chemical Modification
- III. Some Problems Encountered in Chemical Modification
- IV. Analysis of Chemically Modified Proteins
- V. Typical Examples of the Purification of Chemically Modified Proteins
- VI. The Use of Chemical Modification as a Tool for Purification of Proteins
- VII. References
- 6 Chromatographic Peak Shape Analysis
- I. Introduction
- II. Moment Analysis
- III. Experimental Studies
- IV. Slope Analysis
- V. Conclusion
- VI. References
- 7 Sedimentation Equilibrium of Proteins in Density Gradients
- I. Introduction
- II. Theory
- III. Experimental
- IV. Results
- V. References
- 8 Hollow-Fiber Separation Devices and Processes
- I. Introduction
- II. Hollow-Fiber Membranes
- III. Device Configurations
- IV. Dialysis
- V. Combined Dialysis and Concentration
- VI. Concentration
- VII. Drug-Binding Studies
- VIII. Enzyme Reactor
- IX. Conclusion
- X. Appendix
- 9 Affinity Chromatography,Principles and Applications