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140122 ||| eng |
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|a 9781475712575
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| 100 |
1 |
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|a Catsimpoolas, Nicholas
|e [editor]
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| 245 |
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|a Methods of Protein Separation
|h Elektronische Ressource
|c edited by Nicholas Catsimpoolas
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| 250 |
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|a 1st ed. 1975
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| 260 |
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|a New York, NY
|b Springer US
|c 1975, 1975
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| 300 |
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|a XIII, 281 p. 125 illus
|b online resource
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| 505 |
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|a 1 Sedimentation and Gel-Permeation Chromatography of Associating-Dissociating Macromolecules: The Role of Ligand Mediation and Rates of Reaction -- I. Introduction -- II. Theoretical Formulation -- III. Results -- IV. Discussion -- V. References -- 2 Trans Electrophoresis -- I. Introduction -- II. Instrumental Aspects -- III. Data Processing -- IV. TRANS-CZE and TRANS-MZE -- V. TRANS-IF -- VI. Future Developments -- VII. References -- 3 Immunodiffusion -- I. Introduction -- II. Antigen-Antibody Reactions -- III. Immunodiffusion -- IV. Immunodiffusion Combined with Electrophoresis -- V. Helpful Hints -- VI. References -- 4 Isoelectric Focusing in Polyacrylamide Gel -- I. Introduction -- II. Background -- III. Methodology -- IV. Sample Detection -- V. Applications -- VI. Discussion -- VII. References -- 5 Purification of Chemically Modified Proteins -- I. Introduction -- II. General Types and Purposes of Chemical Modification -- III. Some Problems Encountered in Chemical Modification -- IV. Analysis of Chemically Modified Proteins -- V. Typical Examples of the Purification of Chemically Modified Proteins -- VI. The Use of Chemical Modification as a Tool for Purification of Proteins -- VII. References -- 6 Chromatographic Peak Shape Analysis -- I. Introduction -- II. Moment Analysis -- III. Experimental Studies -- IV. Slope Analysis -- V. Conclusion -- VI. References -- 7 Sedimentation Equilibrium of Proteins in Density Gradients -- I. Introduction -- II. Theory -- III. Experimental -- IV. Results -- V. References -- 8 Hollow-Fiber Separation Devices and Processes -- I. Introduction -- II. Hollow-Fiber Membranes -- III. Device Configurations -- IV. Dialysis -- V. Combined Dialysis and Concentration -- VI. Concentration -- VII. Drug-Binding Studies -- VIII. Enzyme Reactor -- IX. Conclusion -- X. Appendix -- 9 Affinity Chromatography,Principles and Applications
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| 653 |
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|a Biochemistry
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 490 |
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|a Biological Separations
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| 028 |
5 |
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|a 10.1007/978-1-4757-1257-5
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| 856 |
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|u https://doi.org/10.1007/978-1-4757-1257-5?nosfx=y
|x Verlag
|3 Volltext
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|a 572
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| 520 |
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|a This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area
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