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Biotechnology Proteins to PCR A Course in Strategies and Lab Techniques

Bibliographic Details
Main Authors: Burden, David W., Whitney, Donald B. (Author)
Format: eBook
Language:English
Published: Boston, MA Birkhäuser 1995, 1995
Edition:1st ed. 1995
Subjects:
Medicine / Research
Life Sciences
Biology / Research
Biotechnology
Biomedical Research
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • 1. Introduction to the Biotechnology Laboratory
  • 1.1Overview
  • 1.2 Background
  • 1.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 2. Introduction to Proteins
  • 2.1 Overview
  • 2.2 Background
  • 2.3 Experimental Design and Procedures
  • Biuret Protein Assay
  • Lowry Protein Assay
  • Study Questions
  • Further Readings
  • 3. Protein Isolation and Preparation of Crude Extract
  • 3.1 Overview
  • 3.2 Background
  • 3.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 4. Batch Purification of Proteins
  • 4.1 Overview
  • 4.2 Background
  • 4.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 5. Protein Purification by Column Chromatography
  • 5.1 Overview
  • 5.2 Background
  • 5.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 6. Protein Analysis and Verification
  • 6.1 Overview
  • 6.2 Background
  • 6.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 7. Designing a Cloning Scheme
  • 7.1 Overview
  • 7.2 Background
  • 73 Experimental Design and Procedures
  • 8. Isolation and Preparation of Nucleic Acids
  • 8.1 Overview
  • 8.2 Background
  • 8.3 Expermental Design and Procedures
  • Study Questions
  • Further Readings
  • 9. Constructing a Gene Bank
  • 9.1 Overview
  • 9.2 Background
  • 9.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 10. Introducing Recombinant Molecules into Escherichia coli
  • 10.1 Overview
  • 10.2 Background
  • 10.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 11. Screening for Clones
  • 11.1 Overview
  • 11.2 Background
  • 11.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 12. Characterizing and Verifying Cloned DNA
  • 12.1 Overview
  • 12.2 Background
  • 12.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 13. Application of DNA Sequence and Clone Data
  • 13.1 Overview
  • 13.2 Background
  • 13.3 Experimental Design and Procedures
  • Study Questions
  • Further Readings
  • 14. The Value of Information

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