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03184nmm a2200337 u 4500 |
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EBX01000000000000000473026 |
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| 008 |
140122 ||| eng |
| 020 |
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|a 9781461242789
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| 100 |
1 |
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|a Burden, David W.
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| 245 |
0 |
0 |
|a Biotechnology Proteins to PCR
|h Elektronische Ressource
|b A Course in Strategies and Lab Techniques
|c by David W. Burden, Donald B. Whitney
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| 250 |
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|a 1st ed. 1995
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| 260 |
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|a Boston, MA
|b Birkhäuser
|c 1995, 1995
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| 300 |
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|a 317 p. 20 illus
|b online resource
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| 505 |
0 |
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|a 1. Introduction to the Biotechnology Laboratory -- 1.1Overview -- 1.2 Background -- 1.3 Experimental Design and Procedures -- Study Questions -- Further Readings -- 2. Introduction to Proteins -- 2.1 Overview -- 2.2 Background -- 2.3 Experimental Design and Procedures -- Biuret Protein Assay -- Lowry Protein Assay -- Study Questions -- Further Readings -- 3. Protein Isolation and Preparation of Crude Extract -- 3.1 Overview -- 3.2 Background -- 3.3 Experimental Design and Procedures -- Study Questions -- Further Readings -- 4. Batch Purification of Proteins -- 4.1 Overview -- 4.2 Background -- 4.3 Experimental Design and Procedures -- Study Questions -- Further Readings -- 5. Protein Purification by Column Chromatography -- 5.1 Overview -- 5.2 Background -- 5.3 Experimental Design and Procedures -- Study Questions -- Further Readings -- 6. Protein Analysis and Verification -- 6.1 Overview -- 6.2 Background -- 6.3 Experimental Design and Procedures -- Study Questions --
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| 505 |
0 |
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|a Further Readings -- 7. Designing a Cloning Scheme -- 7.1 Overview -- 7.2 Background -- 73 Experimental Design and Procedures -- 8. Isolation and Preparation of Nucleic Acids -- 8.1 Overview -- 8.2 Background -- 8.3 Expermental Design and Procedures -- Study Questions -- Further Readings -- 9. Constructing a Gene Bank -- 9.1 Overview -- 9.2 Background -- 9.3 Experimental Design and Procedures -- Study Questions -- Further Readings -- 10. Introducing Recombinant Molecules into Escherichia coli -- 10.1 Overview -- 10.2 Background -- 10.3 Experimental Design and Procedures -- Study Questions -- Further Readings -- 11. Screening for Clones -- 11.1 Overview -- 11.2 Background -- 11.3 Experimental Design and Procedures -- Study Questions -- Further Readings -- 12. Characterizing and Verifying Cloned DNA -- 12.1 Overview -- 12.2 Background -- 12.3 Experimental Design and Procedures -- Study Questions -- Further Readings -- 13. Application of DNA Sequence and Clone Data -- 13.1 Overview --
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| 505 |
0 |
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|a 13.2 Background -- 13.3 Experimental Design and Procedures -- Study Questions -- Further Readings -- 14. The Value of Information
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| 653 |
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|a Medicine / Research
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| 653 |
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|a Life sciences
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| 653 |
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|a Biology / Research
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| 653 |
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|a Life Sciences
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| 653 |
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|a Biotechnology
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| 653 |
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|a Biomedical Research
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| 700 |
1 |
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|a Whitney, Donald B.
|e [author]
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 028 |
5 |
0 |
|a 10.1007/978-1-4612-4278-9
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-4612-4278-9?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
0 |
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|a 660.6
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