Recombinant and In Vitro RNA Synthesis Methods and Protocols
The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the fie...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2012, 2012
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| Edition: | 1st ed. 2012 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold.-Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
- Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR
- General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE
- Preparation of Short RNA by in vitro Transcription
- Native RNA Purification by Gel Filtration Chromatography
- Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5’ and 3’ Ends
- Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends
- Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods.-Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA
- Affinity Purification of RNA using an ARiBo Tag
- Plasmid Template Design and in vitro Transcription of Short RNAs within a ‘Structure Cassette’ for Structure Probing Experiments
- In Vitro Transcription of Modified RNAs
- End-Labeling Oligonucleotides with Chemical Tags After Synthesis
- High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA
- Se-derivatized RNAs for X-ray Crystallography
- Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs
- Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC
- Splint Ligation of RNA with T4 DNA Ligase