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130626 ||| eng |
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|a 9781627031134
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| 100 |
1 |
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|a Conn, Graeme L.
|e [editor]
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| 245 |
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|a Recombinant and In Vitro RNA Synthesis
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Graeme L. Conn
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| 250 |
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|a 1st ed. 2012
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| 260 |
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|a Totowa, NJ
|b Humana
|c 2012, 2012
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| 300 |
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|a XIV, 274 p. 56 illus
|b online resource
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|a Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold.-Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold -- Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR -- General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE -- Preparation of Short RNA by in vitro Transcription -- Native RNA Purification by Gel Filtration Chromatography -- Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5’ and 3’ Ends -- Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends -- Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods.-Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA -- Affinity Purification of RNA using an ARiBo Tag -- Plasmid Template Design and in vitro Transcription of Short RNAs within a ‘Structure Cassette’ for Structure Probing Experiments -- In Vitro Transcription of Modified RNAs -- End-Labeling Oligonucleotides with Chemical Tags After Synthesis -- High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA -- Se-derivatized RNAs for X-ray Crystallography -- Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs -- Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC -- Splint Ligation of RNA with T4 DNA Ligase
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| 653 |
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|a Medical Genetics
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| 653 |
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|a Medical genetics
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| 653 |
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|a Biomaterials
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| 653 |
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|a Nucleic Acid
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| 653 |
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|a Nucleic acids
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b Springer
|a Springer eBooks 2005-
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| 490 |
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|a Methods in Molecular Biology
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| 028 |
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|a 10.1007/978-1-62703-113-4
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| 856 |
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|u https://doi.org/10.1007/978-1-62703-113-4?nosfx=y
|x Verlag
|3 Volltext
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|a 616.042
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| 520 |
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|a The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA.
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