Gel-Free Proteomics Methods and Protocols
Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five c...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2011, 2011
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| Edition: | 1st ed. 2011 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Mass Spectrometry-driven Proteomics: An Introduction
- Metabolic Labeling of Model Organisms using Heavy Nitrogen (15N)
- Trypsin-catalyzed Oxygen-18 Labeling for Quantitative Proteomics
- ICPL Labeling Strategies for Proteome Research
- Quantitative Proteome Analysis using Isobaric Peptide Termini Labeling (IPTL)
- Complete Chemical Modification of Amine and Acid Functional Groups of Peptides and Small Proteins
- Production and Use of Stable-isotope-labeled Proteins for Absolute Quantitative Proteomics
- Organelle Proteomics
- Membrane Protein Digestion – Comparison of LPI Hexalane with Traditional Techniques
- GeLCMS for In-depth Protein Characterization and Advanced Analysis of Proteomes
- Exploring New Proteome Space: Combining Lys-N Proteolytic Digestion and Strong Cation Exchange (SCX) Separation in Peptide Centric MS-driven Proteomics
- Quantitation of Newly Synthesized Proteins by Pulse-labeling with Azidohomoalanine
- Analytical Strategies in Mass Spectrometry Based Phosphoproteomics
- A Protocol on the Use of Titanium Dioxide Chromatography for Phosphoproteomics
- Positional Proteomis at the N-terminus as a Means of Proteome Simplification
- N-terminomics: A High-content Screen for Protease Substrates and their Cleavage Sites
- Protease Specificity Profiling by Tandem Mass Spectrometry Using Proteome-derived Peptide Libraries
- Identification of Proteolytic Products and Natural Protein N-termini by Terminal Amine Isotopic Labeling of Substrates (TAILS)
- Lectins as Tools to Select for Glycosylated Proteins
- Strong Cation Exchange Chromatography for Analysis of Sialylated Glycopeptides
- Titanium Dioxide Enrichment of Sialic Acid-containing Glycopeptides
- Chemical de-O-Glycosylation of Glycoproteins for Applications in LC-based Proteomics
- Ubiquitination and Degradation ofProteins
- Bioinformatics Challenges in Mass Spectrometry Driven Proteomics
- A Case Study on the Comparison of Different Software Tools for Automated Quantification of Peptides