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130626 ||| eng |
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|a 9781617791482
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| 100 |
1 |
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|a Gevaert, Kris
|e [editor]
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| 245 |
0 |
0 |
|a Gel-Free Proteomics
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Kris Gevaert, Joël Vandekerckhove
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| 250 |
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|a 1st ed. 2011
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| 260 |
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|a Totowa, NJ
|b Humana
|c 2011, 2011
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| 300 |
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|a XII, 400 p. 84 illus
|b online resource
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| 505 |
0 |
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|a Mass Spectrometry-driven Proteomics: An Introduction -- Metabolic Labeling of Model Organisms using Heavy Nitrogen (15N) -- Trypsin-catalyzed Oxygen-18 Labeling for Quantitative Proteomics -- ICPL Labeling Strategies for Proteome Research -- Quantitative Proteome Analysis using Isobaric Peptide Termini Labeling (IPTL) -- Complete Chemical Modification of Amine and Acid Functional Groups of Peptides and Small Proteins -- Production and Use of Stable-isotope-labeled Proteins for Absolute Quantitative Proteomics -- Organelle Proteomics -- Membrane Protein Digestion – Comparison of LPI Hexalane with Traditional Techniques -- GeLCMS for In-depth Protein Characterization and Advanced Analysis of Proteomes -- Exploring New Proteome Space: Combining Lys-N Proteolytic Digestion and Strong Cation Exchange (SCX) Separation in Peptide Centric MS-driven Proteomics -- Quantitation of Newly Synthesized Proteins by Pulse-labeling with Azidohomoalanine --
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| 505 |
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|a Analytical Strategies in Mass Spectrometry Based Phosphoproteomics -- A Protocol on the Use of Titanium Dioxide Chromatography for Phosphoproteomics -- Positional Proteomis at the N-terminus as a Means of Proteome Simplification -- N-terminomics: A High-content Screen for Protease Substrates and their Cleavage Sites -- Protease Specificity Profiling by Tandem Mass Spectrometry Using Proteome-derived Peptide Libraries -- Identification of Proteolytic Products and Natural Protein N-termini by Terminal Amine Isotopic Labeling of Substrates (TAILS) -- Lectins as Tools to Select for Glycosylated Proteins -- Strong Cation Exchange Chromatography for Analysis of Sialylated Glycopeptides -- Titanium Dioxide Enrichment of Sialic Acid-containing Glycopeptides -- Chemical de-O-Glycosylation of Glycoproteins for Applications in LC-based Proteomics -- Ubiquitination and Degradation ofProteins -- Bioinformatics Challenges in Mass Spectrometry Driven Proteomics --
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| 505 |
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|a A Case Study on the Comparison of Different Software Tools for Automated Quantification of Peptides
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| 653 |
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|a Protein Biochemistry
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| 653 |
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|a Proteins
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| 700 |
1 |
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|a Vandekerckhove, Joël
|e [editor]
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b Springer
|a Springer eBooks 2005-
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| 490 |
0 |
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|a Methods in Molecular Biology
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| 028 |
5 |
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|a 10.1007/978-1-61779-148-2
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| 856 |
4 |
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|u https://doi.org/10.1007/978-1-61779-148-2?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 572.6
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| 520 |
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|a Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Gel-Free Proteomics: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this critical field
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