RNA Interference Techniques
Non-coding, inhibitory microRNAs have emerged as important modulators of cellular gene expression, through a process called RNA interference (RNAi). To date, hundreds of conserved and species-specific microRNAs have been identified in organisms ranging from single-celled algae to humans. Many of t...
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2011, 2011
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| Edition: | 1st ed. 2011 |
| Series: | Neuromethods
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Designing Hairpin-Based RNAi Shuttles
- Rapid Cloning and Validation of MicroRNA Shuttle Vectors: A Practical Guide
- The Use of Small Non-Coding RNAs to Silence Transcription in Human Cells
- Delivery Strategies for RNAi to the Nervous System
- Cloning Small RNAs
- Profiling the miRNome: Detecting Global miRNA Expression Levels with DNA Microarrays
- High Throughput Profiling of Mature MicroRNA by Real-Time PCR
- In Situ Detection of MicroRNAs in Paraffin Embedded, Formalin Fixed Tissues: Different Methodologies and Co-Localization with Possible Targets
- Tracing of siRNAs Inside Cells by FRET Imaging
- Application of RIP-Chip for the Identification of miRNA Targets