Electron Tomography Methods for Three-Dimensional Visualization of Structures in the Cell

Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with spec...

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Bibliographic Details
Other Authors: Frank, Joachim (Editor)
Format: eBook
Language:English
Published: New York, NY Springer New York 2006, 2006
Edition:2nd ed. 2006
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Principles of Electron Tomography
  • Sample Shrinkage and Radiation Damage of Plastic Sections
  • Electron Tomography of Frozen-hydrated Sections of Cells and Tissues
  • The Electron Microscope as a Structure Projector
  • Cryotomography: Low-dose Automated Tomography of Frozen-hydrated Specimens
  • Fiducial Marker and Hybrid Alignment Methods for Single- and Double-axis Tomography
  • Markerless Alignment in Electron Tomography
  • Algorithms for Three-dimensional Reconstruction From the Imperfect Projection Data Provided by Electron Microscopy
  • Weighted Back-projection Methods
  • Reconstruction With Orthogonal Functions
  • Resolution in Electron Tomography
  • Denoising of Electron Tomograms
  • Segmentation of Three-dimensional Electron Tomographic Images
  • Segmentation of Cell Components Using Prior Knowledge
  • Motif Search in Electron Tomography
  • Localization and Classification of Repetitive Structures in Electron Tomograms of Paracrystalline Assemblies