Cancer Cell Culture Methods and Protocols
The growing of cancer cells in the laboratory has been a powerful tool for studying and understanding the biology of cancer and the effects of drugs on cancer cells. In Cancer Cell Culture: Methods and Protocols, expert researchers describe in detail their most productive and up-to-date methods for...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2004, 2004
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| Edition: | 1st ed. 2004 |
| Series: | Methods in Molecular Medicine
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- to Cancer Cell Culture
- Basic Principles of Cancer Cell Culture
- Essential Techniques of Cancer Cell Culture
- Characterization and Authentication
- Characterization and Authentication of Cancer Cell Lines
- Authentication of Cancer Cell Lines by DNA Fingerprinting
- Cytogenetic Characterization of Tumor Cell Lines
- Isolation and Culture of Specialized Cell Types
- Isolation and Culture of Colon Cancer Cell Lines
- Isolation and Culture of Melanoma Cell Lines
- Isolation and Culture of Human Brain Tumor Cells
- Isolation and Culture of Renal Cancer Cell Lines
- Isolation and Culture of Prostate Cancer Cell Lines
- Isolation and Culture of Ovarian Cancer Cell Lines
- Isolation and Culture of Leukemia Cell Lines
- Functional Assays in Cell Culture
- Cell Sensitivity Assays: Clonogenic Assay
- Cell Sensitivity Assays: The MTT Assay
- PARP Cleavage as a Means of Assessing Apoptosis
- Detection of Apoptosis by the TUNEL Assay
- Apoptosis Measurement by Annexin V Staining
- Cell Adhesion Assays
- Radial Monolayer Cell Migration Assay
- Invasion and Motility Assays
- Identification of Senescence in Cancer Cells
- Angiogenesis Assays
- Flow Cytometric DNA Analysis of Human Cancer Cell Lines
- Modification of Cancer Cells
- DNA-Mediated Gene Transfer
- Development of Drug-Resistant Models
- Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene
- The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts
- Coculture Systems
- Coculture of Prostate Cancer Cells
- Coculture of Ovarian Cells Using Porous Tissue Culture Inserts
- Cell Line Contamination
- Cell Culture Contamination
- Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction
- Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics