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090727 ||| eng |
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|a 9781592594061
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| 050 |
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4 |
|a RC254-282
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| 100 |
1 |
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|a Langdon, Simon P.
|e [editor]
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| 245 |
0 |
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|a Cancer Cell Culture
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Simon P. Langdon
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| 250 |
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|a 1st ed. 2004
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 2004, 2004
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| 300 |
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|a XII, 360 p
|b online resource
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| 505 |
0 |
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|a to Cancer Cell Culture -- Basic Principles of Cancer Cell Culture -- Essential Techniques of Cancer Cell Culture -- Characterization and Authentication -- Characterization and Authentication of Cancer Cell Lines -- Authentication of Cancer Cell Lines by DNA Fingerprinting -- Cytogenetic Characterization of Tumor Cell Lines -- Isolation and Culture of Specialized Cell Types -- Isolation and Culture of Colon Cancer Cell Lines -- Isolation and Culture of Melanoma Cell Lines -- Isolation and Culture of Human Brain Tumor Cells -- Isolation and Culture of Renal Cancer Cell Lines -- Isolation and Culture of Prostate Cancer Cell Lines -- Isolation and Culture of Ovarian Cancer Cell Lines -- Isolation and Culture of Leukemia Cell Lines -- Functional Assays in Cell Culture -- Cell Sensitivity Assays: Clonogenic Assay -- Cell Sensitivity Assays: The MTT Assay -- PARP Cleavage as a Means of Assessing Apoptosis -- Detection of Apoptosis by the TUNEL Assay -- Apoptosis Measurement by Annexin V Staining -- Cell Adhesion Assays -- Radial Monolayer Cell Migration Assay -- Invasion and Motility Assays -- Identification of Senescence in Cancer Cells -- Angiogenesis Assays -- Flow Cytometric DNA Analysis of Human Cancer Cell Lines -- Modification of Cancer Cells -- DNA-Mediated Gene Transfer -- Development of Drug-Resistant Models -- Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene -- The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts -- Coculture Systems -- Coculture of Prostate Cancer Cells -- Coculture of Ovarian Cells Using Porous Tissue Culture Inserts -- Cell Line Contamination -- Cell Culture Contamination -- Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction -- Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics
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| 653 |
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|a Oncology
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
0 |
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|a Methods in Molecular Medicine
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| 024 |
8 |
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|a 10.1385/1592594069
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| 856 |
4 |
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|u https://doi.org/10.1385/1592594069?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 616.994
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| 520 |
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|a The growing of cancer cells in the laboratory has been a powerful tool for studying and understanding the biology of cancer and the effects of drugs on cancer cells. In Cancer Cell Culture: Methods and Protocols, expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. Suitable for novice and experienced researchers alike, these readily reproducible techniques solve a diverse range of experimental problems. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the coculture of different cell types; and the detection and treatment of contamination. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Cancer Cell Culture: Methods and Protocols provides both basic scientists and clinical researchers with a gold-standard collection of advanced techniques for culturing cancer cells effectively in their laboratories
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