Peptide Nucleic Acids Methods and Protocols
Peptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either alrea...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2002, 2002
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| Edition: | 1st ed. 2002 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- PNA Technology
- Chemistry
- Solid Phase Synthesis of PNA Oligomers
- Synthesis of PNA-Peptide Conjugates
- Parallel Synthesis of PNA-Peptide Conjugate Libraries
- Thermodynamics of PNA Interactions with DNA and RNA
- PNA Technology
- Measurement of PNA Binding to Double-Stranded DNA
- PNA-Mediated Immobilization of Supercoiled DNA
- PNA Openers and Their Applications
- Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes
- Diagnostics
- PNA-Mediated PCR Clamping
- PNA Clamping Techniques for the Determination of Oncogene Mutations
- PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis
- Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices
- Antisense and Antigene
- Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells
- Synthesis of Cell-Penetrating Peptide-PNA Constructs
- Antisense Inhibition of Bacterial Gene Expression and Cell Growth
- In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops
- Animal Studies
- In Vitro and In Vivo Studies on the Pharmacokinetics and Metabolism of PNA Constructs in Rodents