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090727 ||| eng |
| 020 |
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|a 9781592592906
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| 050 |
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4 |
|a QD415-436
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| 100 |
1 |
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|a Nielsen, Peter E.
|e [editor]
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| 245 |
0 |
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|a Peptide Nucleic Acids
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Peter E. Nielsen
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| 250 |
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|a 1st ed. 2002
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 2002, 2002
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| 300 |
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|a X, 274 p
|b online resource
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| 505 |
0 |
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|a PNA Technology -- Chemistry -- Solid Phase Synthesis of PNA Oligomers -- Synthesis of PNA-Peptide Conjugates -- Parallel Synthesis of PNA-Peptide Conjugate Libraries -- Thermodynamics of PNA Interactions with DNA and RNA -- PNA Technology -- Measurement of PNA Binding to Double-Stranded DNA -- PNA-Mediated Immobilization of Supercoiled DNA -- PNA Openers and Their Applications -- Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes -- Diagnostics -- PNA-Mediated PCR Clamping -- PNA Clamping Techniques for the Determination of Oncogene Mutations -- PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis -- Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices -- Antisense and Antigene -- Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells -- Synthesis of Cell-Penetrating Peptide-PNA Constructs -- Antisense Inhibition of Bacterial Gene Expression and Cell Growth -- In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops -- Animal Studies -- In Vitro and In Vivo Studies on the Pharmacokinetics and Metabolism of PNA Constructs in Rodents
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| 653 |
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|a Biochemistry
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
0 |
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|a Methods in Molecular Biology
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| 024 |
8 |
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|a 10.1385/1592592902
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| 856 |
4 |
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|u https://doi.org/10.1385/1592592902?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 572
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| 520 |
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|a Peptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on experience in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process. Up-to-date and highly practical, Peptide Nucleic Acids: Methods and Protocols provides for both novice and experienced researchers a thorough and easy introduction to the optimal use of today's PNA technology across a wide variety of fields, including molecular biology, medicinal chemistry, genetic diagnostics, and drug development
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