Protein Sequencing Protocols
Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current im...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2003, 2003
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| Edition: | 2nd ed. 2003 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- D-Amino Acid Analysis
- Validation of Amino Acid Analysis Methods
- Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins
- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry
- Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes
- Direct Identification of Proteins in Ultracomplex Mixtures
- Identification of PTH-Amino Acids by HPLC
- Protein Sequencer Maintenance and Troubleshooting
- N-Terminal Protein Sequencing for Special Applications
- Identification of Phosphorylation Sites by Edman Degradation
- Validation of Protein Sequencing in a Regulated Laboratory
- Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method
- Chemical Modifications of Proteins as an Aid to Sequence Analysis
- Deblocking of N-Terminally Modified Proteins
- Deblocking of Proteins Containing N-Terminal PyroglutamicAcid
- Strategies for Handling Polypeptides on a Micro-Scale
- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing
- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization
- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization
- Enzymatic Cleavage of Proteins
- Chemical Cleavage of Polypeptides
- Enzymatic Preparation and Isolation of Glycopeptides
- Analytical and Micropreparative Capillary Electrophoresis of Peptides
- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues
- Hydrolysis of Samples for Amino Acid Analysis
- Amino Acid Analysis
- Post Column Amino Acid Analysis
- Amino Acid Analysis Using Pre-Column Derivatization with 6-AminoquinolylN-Hydroxysuccinimidyl Carbamate
- Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection
- Identification of Sites of Glycosylation
- Analysis of Sites of Protein Phosphorylation
- Quantitation and Location of Disulfide Bonds in Proteins
- Getting the Most from Your Protein Sequence
- Letter Codes, Structures, Masses, and Derivatives of Amino Acids
- Protein Consensus Sequence Motifs