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090727 ||| eng |
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|a 9781592593422
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4 |
|a QD415-436
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| 100 |
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|a Smith, Bryan John
|e [editor]
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| 245 |
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|a Protein Sequencing Protocols
|h Elektronische Ressource
|c edited by Bryan John Smith
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| 250 |
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|a 2nd ed. 2003
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 2003, 2003
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| 300 |
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|a XII, 493 p
|b online resource
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|a D-Amino Acid Analysis -- Validation of Amino Acid Analysis Methods -- Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins -- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes -- Direct Identification of Proteins in Ultracomplex Mixtures -- Identification of PTH-Amino Acids by HPLC -- Protein Sequencer Maintenance and Troubleshooting -- N-Terminal Protein Sequencing for Special Applications -- Identification of Phosphorylation Sites by Edman Degradation -- Validation of Protein Sequencing in a Regulated Laboratory -- Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method -- Chemical Modifications of Proteins as an Aid to Sequence Analysis -- Deblocking of N-Terminally Modified Proteins -- Deblocking of Proteins Containing N-Terminal PyroglutamicAcid --
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|a Strategies for Handling Polypeptides on a Micro-Scale -- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing -- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization -- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization -- Enzymatic Cleavage of Proteins -- Chemical Cleavage of Polypeptides -- Enzymatic Preparation and Isolation of Glycopeptides -- Analytical and Micropreparative Capillary Electrophoresis of Peptides -- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues -- Hydrolysis of Samples for Amino Acid Analysis -- Amino Acid Analysis -- Post Column Amino Acid Analysis -- Amino Acid Analysis Using Pre-Column Derivatization with 6-AminoquinolylN-Hydroxysuccinimidyl Carbamate -- Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection --
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| 505 |
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|a Identification of Sites of Glycosylation -- Analysis of Sites of Protein Phosphorylation -- Quantitation and Location of Disulfide Bonds in Proteins -- Getting the Most from Your Protein Sequence -- Letter Codes, Structures, Masses, and Derivatives of Amino Acids -- Protein Consensus Sequence Motifs
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| 653 |
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|a Biochemistry
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| 041 |
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|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
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|a Methods in Molecular Biology
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| 024 |
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|a 10.1385/1592593429
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| 856 |
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|u https://doi.org/10.1385/1592593429?nosfx=y
|x Verlag
|3 Volltext
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|a 572
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| 520 |
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|a Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to the analysis of protein sequence information via the powerful new tools of bioinformatics. Each readily reproducible protocol contains detailed step-by-step instructions to ensure successful experimental results, along with notes on troubleshooting and how to avoid pitfalls. Valuable appendices summarize changes in mass arising from protein modification, as well as consensus sequences for protein modification. Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination and thus help assure robust results
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