Developmental Biology Protocols Volume II

The 1st and 3rd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods...

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Bibliographic Details
Other Authors: Tuan, Rocky S. (Editor), Lo, Cecilia W. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2000, 2000
Edition:1st ed. 2000
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Developmental Biology Protocols
  • Organogenesis
  • Drosophila as a Genetic Tool to Define Vertebrate Pathway Players
  • Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis
  • Analysis of Mammary Gland Morphogenesis
  • Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro
  • Craniofacial Development and Patterning
  • Craniofacial Skeletel Morphogenesis In Vitro
  • Skeletal Morphogenesis
  • Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration
  • Retroviral Infection of T-Cell Precursors in Thymic Organ Culture
  • Assay for the Isolation of Hepatogenic Factors
  • Skin Morphogenesis
  • Apoptosis in Development
  • Methods to Detect Patterns of Cell Death in Drosophila
  • Abnormal Development and Teratology
  • Mechanisms of Neurulation
  • Neurulation and Neural Tube Closure Defects
  • Neural Tube Defects
  • Experimental Manipulation and Morphometric Analysis of Neural Tube Development
  • HPLC-Based mRNA Differential Display
  • Production of Transgenic Drosophila
  • Transgenic Manipulation of the Sea Urchin Embryo
  • Transgenic Zebrafish
  • Production of Avian Chimeras and Germline Transmission
  • Incorporation of Genetically Modified Cells in Chicken Chimeras
  • Long-Term Culture of Chicken Blastodermal Cells (CBCs) and Selection of Transfected CBCs Using Antibiotic Resistance
  • Nuclear Transplantation and Cloning in Mammals
  • Production of Transgenic Mice
  • Production of Transgenic Mice with Yeast Artificial Chromosomes
  • Gene-Targeting Strategies
  • Chimeric Animals and GermlineTransmission
  • Conditional Gene Knockout Using Cre Recombinase
  • Application of Cre/loxP in Drosophila
  • Isolation of Neuroepithelium and Formation of Minispheres
  • Examination of Normal and Abnormal Placentation in the Mouse
  • Palatal Dysmorphogenesis
  • Palatal Dysmorphogenesis
  • Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities
  • Assessment of Laterality Defects in Rodent Embryos
  • Cardiac Morphogenesis and Dysmorphogenesis I. Normal Development
  • Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart
  • Biologically Based Risk Assessment Models for Developmental Toxicity
  • Screening and Mapping of Novel Genes and Mutations
  • Positional Cloning
  • Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in the Mouse
  • PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes
  • Analysis of mRNA Expression During Preimplantation Development
  • Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Embryos