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090727 ||| eng |
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|a 9781592590650
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| 050 |
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4 |
|a QH491-492
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| 100 |
1 |
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|a Tuan, Rocky S.
|e [editor]
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| 245 |
0 |
0 |
|a Developmental Biology Protocols
|h Elektronische Ressource
|b Volume II
|c edited by Rocky S. Tuan, Cecilia W. Lo
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| 250 |
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|a 1st ed. 2000
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 2000, 2000
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| 300 |
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|a XXV, 505 p
|b online resource
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| 505 |
0 |
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|a Developmental Biology Protocols -- Organogenesis -- Drosophila as a Genetic Tool to Define Vertebrate Pathway Players -- Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis -- Analysis of Mammary Gland Morphogenesis -- Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro -- Craniofacial Development and Patterning -- Craniofacial Skeletel Morphogenesis In Vitro -- Skeletal Morphogenesis -- Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration -- Retroviral Infection of T-Cell Precursors in Thymic Organ Culture -- Assay for the Isolation of Hepatogenic Factors -- Skin Morphogenesis -- Apoptosis in Development -- Methods to Detect Patterns of Cell Death in Drosophila -- Abnormal Development and Teratology -- Mechanisms of Neurulation -- Neurulation and Neural Tube Closure Defects -- Neural Tube Defects -- Experimental Manipulation and Morphometric Analysis of Neural Tube Development --
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| 505 |
0 |
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|a HPLC-Based mRNA Differential Display -- Production of Transgenic Drosophila -- Transgenic Manipulation of the Sea Urchin Embryo -- Transgenic Zebrafish -- Production of Avian Chimeras and Germline Transmission -- Incorporation of Genetically Modified Cells in Chicken Chimeras -- Long-Term Culture of Chicken Blastodermal Cells (CBCs) and Selection of Transfected CBCs Using Antibiotic Resistance -- Nuclear Transplantation and Cloning in Mammals -- Production of Transgenic Mice -- Production of Transgenic Mice with Yeast Artificial Chromosomes -- Gene-Targeting Strategies -- Chimeric Animals and GermlineTransmission -- Conditional Gene Knockout Using Cre Recombinase -- Application of Cre/loxP in Drosophila
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| 505 |
0 |
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|a Isolation of Neuroepithelium and Formation of Minispheres -- Examination of Normal and Abnormal Placentation in the Mouse -- Palatal Dysmorphogenesis -- Palatal Dysmorphogenesis -- Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities -- Assessment of Laterality Defects in Rodent Embryos -- Cardiac Morphogenesis and Dysmorphogenesis I. Normal Development -- Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart -- Biologically Based Risk Assessment Models for Developmental Toxicity -- Screening and Mapping of Novel Genes and Mutations -- Positional Cloning -- Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in the Mouse -- PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes -- Analysis of mRNA Expression During Preimplantation Development -- Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Embryos --
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| 653 |
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|a Developmental biology
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| 653 |
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|a Developmental Biology and Stem Cells
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| 700 |
1 |
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|a Lo, Cecilia W.
|e [editor]
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
0 |
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|a Methods in Molecular Biology
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| 024 |
8 |
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|a 10.1385/1592590659
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| 856 |
4 |
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|u https://doi.org/10.1385/1592590659?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
0 |
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|a 571.8
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| 520 |
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|a The 1st and 3rd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors.
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| 520 |
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|a The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 2nd volume of three describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts.
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| 520 |
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|a No less innovative, volume 3 introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis
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