PCR Mutation Detection Protocols
Application of DNA technology to the identification of disease-causing mutations has become widespread in recent years. PCR Mutation Detection Protocols, provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable fo...
| Other Authors: | , |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2002, 2002
|
| Edition: | 1st ed. 2002 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Agarose and Polyacrylamide Gel Electrophoresis
- Internal Labeling of DNA Probes
- End-Labeling of DNA Probes
- Southern Blotting of Agarose Gels by Capillary Transfer
- Restriction Fragment Length Polymorphism
- PCR
- Allele-Specific Oligonucleotide PCR
- Long-Range PCR
- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
- Cycle Sequencing of PCR Products
- Fluorescent In Situ Hybridization
- The Protein Truncation Test
- Mutation Detection in Factor VIII cDNA from Lymphocytes of Hemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch
- Denaturing Gradient Gel Electrophoresis
- Conformation-Sensitive Gel Electrophoresis
- SSCP/Heteroduplex Analysis
- Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection
- Automated Genotyping Using the DNA MassArray™ Technology
- An Introduction to Bioinformatics