PCR Mutation Detection Protocols

Application of DNA technology to the identification of disease-causing mutations has become widespread in recent years. PCR Mutation Detection Protocols, provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable fo...

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Bibliographic Details
Other Authors: Theophilus, Bimal D. M. (Editor), Rapley, Ralph (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2002, 2002
Edition:1st ed. 2002
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
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505 0 |a Agarose and Polyacrylamide Gel Electrophoresis -- Internal Labeling of DNA Probes -- End-Labeling of DNA Probes -- Southern Blotting of Agarose Gels by Capillary Transfer -- Restriction Fragment Length Polymorphism -- PCR -- Allele-Specific Oligonucleotide PCR -- Long-Range PCR -- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing -- Cycle Sequencing of PCR Products -- Fluorescent In Situ Hybridization -- The Protein Truncation Test -- Mutation Detection in Factor VIII cDNA from Lymphocytes of Hemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch -- Denaturing Gradient Gel Electrophoresis -- Conformation-Sensitive Gel Electrophoresis -- SSCP/Heteroduplex Analysis -- Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection -- Automated Genotyping Using the DNA MassArray™ Technology -- An Introduction to Bioinformatics 
653 |a Cell Biology 
653 |a Cytology 
700 1 |a Rapley, Ralph  |e [editor] 
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520 |a Application of DNA technology to the identification of disease-causing mutations has become widespread in recent years. PCR Mutation Detection Protocols, provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Additional coverage is given to the direct use of DNA sequencing as a detection method in its own right and to the characterization of mutations previously located by other screening techniques. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution. Comprehensive and highly practical, PCR Mutation Detection Protocols assures both seasoned and novice investigators access to the highly productive and readily reproducible PCR-based mutation detection methods, techniques that are laying the groundwork for many of today's major scientific and medical advances