Epigenome Editing Methods and Protocols
This second volume details new and updated methods covering applications of epigenome editing. Chapters guide readers through general and topical reviews, DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2024, 2024
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| Edition: | 2nd ed. 2024 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Development of locus-directed editing of the epigenome from basic mechanistic engineering to first clinical applications
- Designing epigenome editors: Considerations of biochemical and locus specificities
- Fine-Tuning the Epigenetic Landscape: Chemical Modulation of Epigenome Editors
- Using high-throughput measurements to identify principles of transcriptional and epigenetic regulators
- Reader-Effectors as actuators of epigenome editing
- Neuroepigenetic editing
- Optimized protocol for the regulation of DNA methylation and gene expression using modified dCas9-SunTag platforms
- Protocol for DNA methylation editing of imprinted loci and assessment of the effects
- Protocol for allele specific epigenome editing using CRISPR/dCas9
- Plant epigenetic editing to analyze the function of histone modifications in gene-specific regulation
- Protocol for efficient generation of chimeric antigen receptor T cells with multiplexed gene silencing by epigenome editing
- Fluorescent reporter systems to investigate chromatin effector proteins in living cells: Applications and protocol
- Plasmid delivery and single-cell plasmid expression analysis for CRISPR/dCas9-based epigenetic editing