Cryo-Electron Tomography Structural Biology in situ
This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facili...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Cham
Springer International Publishing
2024, 2024
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| Edition: | 1st ed. 2024 |
| Series: | Focus on Structural Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- 1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography
- 2. Principles of tomographic reconstruction
- 3. Cryo-preparation for cellular tomography
- 4. Cryo-FIB milling
- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques
- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM
- 7. Data collection
- 8. Subvolume averaging and classification
- 9. High resolution tomography/ Molecular model building and visualization
- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography
- 11. Tomogram segmentation
- 12. Electron tomography of cryo-fixed and resin-embedded samples
- 13. Democratization of tomographic data / FAIR data