Embryo Models In Vitro Methods and Protocols
This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspe...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2024, 2024
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| Edition: | 1st ed. 2024 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Generation of Human Blastoids from Naïve Pluripotent Stem Cells
- Human Pre-Gastrulation Embryo Culture in 3D Condition
- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System
- Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models
- Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology
- Endometrial Assembloids to Model Human Embryo Implantation In Vitro
- Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (µPASE)
- Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells
- Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells
- Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells
- Generation of Stem Cell-Based Mouse Embryo-Like Structures
- Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond
- Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts
- Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast
- Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos
- Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas
- Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL
- ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis
- Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence
- Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics
- Whole-Mount RNA, Single Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos