Bacteriophages Methods and Protocols

This volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small to jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different envir...

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Bibliographic Details
Other Authors: Tumban, Ebenezer (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2024, 2024
Edition:1st ed. 2024
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Structural and genomic diversity of bacteriophages
  • The diversity of bacteriophages in human gut
  • Breaking the ice - A review of phages from polar ecosystems
  • The diversity of bacteriophages in hot spring
  • Isolation of bacteriophage from soil samples in a poorly equipped field lab in Kruger National Park
  • Purification and up-concentration of bacteriophages and viruses from fecal samples
  • Isolation of enterococcus phages from municipal wastewater samples using an enrichment step
  • Phage DNA extraction, genome assembly, and genome closure
  • Enumeration of bacteriophages by plaque assay
  • Detection and quantification of phages in wastewater samples by culture and molecular methods
  • Flow virometry: A fluorescence-based approach to enumerate bacteriophages in liquid samples
  • A metagenomics approach to enumerate bacteriophages in a Food Niche
  • Bioinformatic analysis of Staphylococcus phages: a key step for safe cocktail development
  • Use of localized reconstruction to visualize the Shigella-bacteriophage Sf6 tail apparatus
  • Bacteriophage-host interactions and co-evolution
  • Unraveling physical interactions of Clostridioides difficile with phage and phage-derived proteins using in vitro and whole-cell assays
  • Phage Transduction of Staphylococcus aureus
  • The Next Generation of Drug Delivery: Harnessing the Power of Bacteriophages
  • Construction of nonnatural cysteine-crosslinked phage libraries
  • Application of next generation sequencing in phage display
  • The application of bacteriophage and photoacoustic flow cytometry in bacterial identification
  • Propagation, purification and characterization of bacteriophages for phage
  • Overcoming bacteriophage resistance in phage therapy
  • .Bacteriophage virus-like particles: platforms for vaccine design