Vascular Hyperpermeability Methods and Protocols

This volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in...

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Bibliographic Details
Other Authors: Tharakan, Binu (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2024, 2024
Edition:1st ed. 2024
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Imaging and Analysis of the Dynamics of Filamentous Actin Structures in Live Endothelial Cells
  • Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs)
  • Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification
  • Measurement of Blood-Brain Barrier Hyperpermeability Using Evans Blue Extravasation Assay
  • Assessment of Endothelial Barrier Functions in Extra Embryonic Vasculature of Chick Embryo as an Alternative Model
  • Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells
  • An In Vitro Bilayer Model of Human Primary Retinal Pigment Epithelial and Choroid Endothelial Cells for Permeability Studies
  • Quantifying Adhesion of Inflammatory Cells to the Endothelium In Vitro
  • Determination of Tight Junction Integrity in Brain Endothelial Cells Based on Tight Junction Protein Expression
  • Evaluation of Glycolysis and Mitochondrial Function in Endothelial Cells Using the Seahorse Analyzer
  • Determination of Solute Permeability of Microvascular Endothelial Cell Monolayers In Vitro
  • Evaluation of Vascular Permeability in Inflamed Vessels of the Cremaster Muscle in Live Mice
  • Lymphatic Vascular Permeability Determined from Direct Measurements of Solute Flux
  • Evaluation of Mesenteric Microvascular Hyperpermeability Following Hemorrhagic Shock using Intravital Microscopy
  • Determination of Endothelial Barrier Resistance by Electric Cell-Substrate Impedance Sensing (ECIS) System
  • Time-Lapse Observation of Cell Dynamics during Angiogenesis Using the Rat Mesentery Culture Model
  • Evaluation of Barrier Integrity using a Two-Layered Microfluidic Device Mimicking the Blood-Brain Barrier
  • Intravital Imaging of Leukocyte-Endothelial Interaction in Hindlimb Ischemia/Reperfusion Injury by Intravital Multiphoton Microscopy
  • Studying Angiogenesis using Matrigel In Vitro and In Vivo
  • Determination of Blood-Brain Barrier Hyperpermeability Using Intravital Microscopy
  • Evaluation of Tight Junction Integrity in Brain Endothelial Cells using Confocal Microscopy