Cell-Secreted Vesicles Methods and Protocols

This detailed volume presents hands-on technological protocols used to target an array of cell-secreted extracellular vesicles (EVs) in a variety of biological systems. Beginning with methods for EV purification and analysis, the book continues with sections on the study of EV functions as well as s...

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Bibliographic Details
Other Authors: Vainio, Seppo (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2023, 2023
Edition:1st ed. 2023
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • A Novel Assay for Investigating the Role of Exosomes in Tumor Cell-Endothelial Cell Crosstalk
  • Nanoparticle (NP) Loading by Direct Incubation with Extracellular Vesicles-Secretor Cells: NP Encapsulation and Exosome Characterization
  • Two Complementary Strategies to Quantitate Extracellular Vesicles Uptake Using Bioluminescence and Non-Lipidic Dyes
  • Integrin-Mediated Exosomal Homing to Organs
  • Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research
  • Quantitative Analysis of Extracellular Vesicle Release Using Artificial MicroRNAs
  • Purification of Bacterial-Enriched Extracellular Vesicle Samples from Feces by Density Gradient Ultracentrifugation
  • Isolation and Characterization of Extracellular Vesicles from Lymphocytes
  • Biogenesis of Mesoporous Silica Nanoparticles Enclosed in Extracellular Vesicles by Mouse Renal Adenocarcinoma Cells
  • Plasmon-Enhanced Characterization of Single Extracellular Vesicles
  • Detection of Cell-Derived Exosomes via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles
  • In Vivo Analysis of Heterogeneous Extracellular Vesicles Using a Red-Shifted Bioluminescence Resonance Energy Transfer Reporter Protein
  • Characterization of Extracellular Vesicles by Transmission Electron Microscopy and Immunolabelling Electron Microscopy
  • Extracellular Vesicles Isolation by a Tangential-Flow-Filtration-Based Large-Scale Purification Method
  • Metabolomics Analysis of Urinary Extracellular Vesicles by Nuclear Magnetic Resonance and Liquid Chromatography-Mass Spectrometry
  • Methodologies for Scalable Production of High Quality Purified Small Extracellular Vesicles from Conditioned Medium
  • Automated Online Isolation and Fractionation Method for Subpopulations of Extracellular Vesicles
  • Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications
  • Cilia-Derived Extracellular Vesicles in Caenorhabditis elegans: In Vivo Imaging and Quantification of Extracellular Vesicles Release and Capture
  • Exosome-Based COVID-19 Vaccine