Cancer Cell Culture Methods and Protocols

This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional cancer cell culture, cell model...

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Bibliographic Details
Other Authors: Movia, Dania (Editor), Prina-Mello, Adriele (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2023, 2023
Edition:1st ed. 2023
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Cancer Cell Culture: The Basics and Two-Dimensional Cultures
  • Cell Cultures at the Air–Liquid Interface and Their Application in Cancer Research
  • Three-Dimensional Spheroids for Cancer Research
  • Disease Animal Models for Cancer Research
  • Generation of Radio-Resistant Prostate Cancer Cells
  • Generation and Characterization of an Isogenic Cell Line Model of Radioresistant Oesophageal Adenocarcinoma
  • Developing an In Vitro Isogenic Model of Chemotherapy-Resistant Lung Cancer
  • Culturing Human Lung Adenocarcinoma Cells in a Serum-Free Environment
  • A Method for Culturing 3D Tumoroids of Lung Adenocarcinoma Cells at the Air-Liquid Interface
  • Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cancer Patients
  • Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research
  • A Self-Assembly Method for Creating Vascularized Tumor Explants Using Biomaterials for 3D Culture
  • A Step-by-Step Methodological Guide for Developing Zonal Multicellular Scaffold-Based Pancreatic Cancer Models
  • Measuring Immune Cell Movement Towards the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay
  • A Method for the In Vitro Cytotoxicity Assessment of Anti-Cancer Compounds and Materials Using High Content Screening Analysis
  • Testing the Effects of Magnetic Hyperthermia in In Vitro 2D Tumor Cultures
  • Cell Viability Assay with 3D Prostate Tumor Spheroids
  • Analysis of Cancer Cell Line Secretomes: A Complementary Source of Disease-Specific Protein Biomarkers