Advanced Methods in Structural Biology
This volume explores the latest integrated bioprocesses and technologies used to study the production of the target recombinant protein of therapeutic or diagnostic interest; its isolation, purification, and stabilization; and the bio-interaction and structural analyses. The chapters in this book ar...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2023, 2023
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| Edition: | 1st ed. 2023 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Biophysical Characterization of Membrane Proteins
- Expression in Saccharomyces cerevisiae and Purification of a Human Phospholipid Flippase
- Preparation of Protein-Enriched Outer Membrane Vesicles from Escherichia Coli for In Situ Structural Biology of Outer Membrane Proteins
- Receptor-Based Virtual Screening of Large Libraries in a Multi-Level in Silico Approach
- Protein-Protein Interaction Studies Using Molecular Dynamics Simulation
- Isolation of Bacterial Extracellular Vesicles and Identification of their Protein Cargo
- Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry
- Protein Interaction Analysis by Surface Plasmon Resonance
- High-Throughput Protein Crystallization in an Integrated Droplet-Based Microfluidic Platform
- Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations
- Using Small-Angle X-Ray Scattering to Characterize Biological Systems:A General Overview and Practical Tips
- Cloning and Recombinant Protein Expression in Lactococcus lactis
- E6 Tagged Protein Production, Extraction, and Purification from Escherichia coli Lysate
- Mini-Bioreactor Platform for Membrane Proteins Production in Komagataella pastoris
- Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS)
- High-Throughput Production and Optimization of Membrane Proteins after Expression in Mammalian Cells
- Fluorescent Fusion Proteins Expression in Plant Cells
- Functional Expression of the Ectodomain of Plant Receptor Kinases in Plant Suspension Culture
- A Simple Purification Method for Heat Stable Recombinant Low Molecular Weight Proteins and Peptides Via GST-Fusion Products
- eGFP as an All-in-One Tag for Purification of Membrane Proteins
- Effects of Ionic Liquids as Additives on Protein Crystallization
- Screening of Buffers and Additives for Protein Stabilization by Thermal Shift Assay: A Practical Approach
- Nuclear Magnetic Resonance-Guided Structural Analysis of Moderate-Affinity Protein Complexes with Intrinsically Disordered Polypeptides
- Protein Structural Analysis by Cryogenic Electron Microscopy