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230201 ||| eng |
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|a 9783031194856
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| 100 |
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|a Mani, Shalini
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| 245 |
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|a Animal Cell Culture: Principles and Practice
|h Elektronische Ressource
|c by Shalini Mani, Manisha Singh, Anil Kumar
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| 250 |
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|a 1st ed. 2023
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| 260 |
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|a Cham
|b Springer International Publishing
|c 2023, 2023
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| 300 |
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|a XVI, 327 p. 91 illus., 85 illus. in color
|b online resource
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| 505 |
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|a 1. Overview to animal cell culture -- 2. Cell culture laboratory -- 3. Good lab practices and bio-safety measures -- 4. Managing sterility in the lab -- 5. Media and buffer preparation for cell culture -- 6. Properties of cultured cells and selection of culture media -- 7. Selection and maintenance of cultured cells -- 8. Inoculation and passaging of adherent cells -- 9. Counting of cells -- 10. Cryopreservation of cell lines -- 11. Resuscitation of frozen cell line -- 12. Isolation and culturing of cells from different tissues -- 13. Differentiation, transformation and immortalization of cells -- 14. Co-culture techniques -- 15. 3d cell culture techniques -- 16. Stem cell culture techniques -- 17. Identification and removal of biological contamination in the media and cell suspensions -- 18. Analysis of cell growth kinetics in suspension and adherent types cell line -- 19. Applications of animal cell culture based assays -- 20. Ethical issues in animal cell culture -- 21. Common troubleshooting methods in cell culture techniques
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| 653 |
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|a Cell Biology
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| 653 |
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|a Microbiology / Technique
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| 653 |
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|a Experimental immunology
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| 653 |
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|a Cytology
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| 653 |
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|a Immunological Techniques
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| 653 |
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|a Cytological Techniques
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| 653 |
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|a Microbiology Techniques
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| 653 |
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|a Biotechnology
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| 653 |
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|a Cytology / Technique
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| 653 |
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|a Biology / Technique
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| 700 |
1 |
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|a Singh, Manisha
|e [author]
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| 700 |
1 |
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|a Kumar, Anil
|e [author]
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b Springer
|a Springer eBooks 2005-
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| 490 |
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|a Techniques in Life Science and Biomedicine for the Non-Expert
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| 028 |
5 |
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|a 10.1007/978-3-031-19485-6
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| 856 |
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|u https://doi.org/10.1007/978-3-031-19485-6?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 571.6028
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| 520 |
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|a This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more.
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