Chemokine-Glycosaminoglycan Interactions Methods and Protocols

This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo mo...

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Bibliographic Details
Other Authors: Lucas, Alexandra R. (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2023, 2023
Edition:1st ed. 2023
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy
  • Remote Ischemic Pre-Conditioning (RIPC)
  • Mouse Corneal Transplantation
  • Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant
  • Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine:GAG Interaction and Therapeutic Blockade
  • Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion
  • In Vitro (Trans)migration Experiment Using Chemokines as Stimulatory Factor
  • The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins
  • Flow Cytometry Analysis of Immune Cell Responses
  • Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence
  • Protein Microarrays and Their Fabrication
  • NMR Methods for Characterization of Glycosaminoglycan-Chemokine Interactions
  • Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG-Protein Interactions
  • Isolation and Compositional Analysis of Glycosaminoglycans
  • How to Design Peptides
  • Methods to Assess Chemokine Binding and Anti-Chemotactic Activity of Virus Proteins
  • Producing Biologics with Defined N-Glycosylation in Plants
  • Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery