HDAC/HAT Function Assessment and Inhibitor Development Methods and Protocols
This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel a...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2023, 2023
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| Edition: | 2nd ed. 2023 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Analyzing Lymphoma Development and Progression Using HDACi in Mouse Models
- Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells
- Defined Human Leukemic CD34+ Liquid Cultures to Study HDAC/Transcriptional Repressor Complexes
- Development of a Cellular Model Mimicking Specific HDAC Inhibitors
- Evaluation of Anti-Tumor and On-Target Activity of HDAC Inhibitors with the Zebrafish Embryo Xenograft Model
- Human Platelet Lysate as Valid Cell Growth Additive to Assess Protein Acetylation
- Investigating Physiopathological Roles for Sirtuins in a Mouse Model
- Patient-Derived Organoids for In Vivo Validation of In Vitro Data
- Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges
- Development of Pyrazine-Anilinobenzamides as Histone Deacetylase HDAC1-3 Selective Inhibitors and Biological Testing Against Pancreas Cancer Cell Lines
- Evaluation of Small Molecule HDAC Inhibitors through In Vitro and In Cellulo Approaches
- Monitoring Changes in Intracellular Reactive Oxygen SpeciesLevels in Response to Histone Deacetylase Inhibitors
- Single-Cell Analysis of Histone Acetylation Dynamics at Replication Forks Using PLA and SIRF
- Testing the Effect of Histone Acetyltransferases on Local Chromatin Compaction
- A Genome-Wide CRISPR-Cas9 Loss-of-Function Screening to Identify Host Restriction Factors Modulating Oncolytic Virotherapy
- Cloning Strategy for HDAC1/HDAC2 Hybrid Protein Expression in Mammalian Cells
- Continuous Histone Deacylase Activity Assays
- CRISPR Activation/Interference Screen to Identify Genetic Networks in HDAC-Inhibitor Resistant Cells
- Determining Potency of Inhibitors Targeting Histone Deacetylase 6 by Quantification of Acetylated Tubulin in Cells
- Expression and Crystallization of HDAC6 Tandem Catalytic Domains
- Fluorescence Polarization-Based Competition Assays to Evaluate Histone Deacetylase 6 Inhibitors
- Immunoprecipitation of HDAC6 and Interacting Proteins
- Synthesis, Biochemical, and Cellular Evaluation of HDAC6 Targeting Proteolysis Targeting Chimeras
- HDACi Delivery Systems Based on Cellulose Valproate Nanoparticles
- Synthesis and Characterization of Reversible Covalent HDAC4 Inhibitors
- Analysis of Cell Cycle and DNA Compaction Dependent Subnuclear Distribution of Histone Marks
- Analyzing the Effects of HDAC Inhibitors on DNA Damage and Associated Cytotoxicity in Primary Hepatocytes
- Assessment of HDAC Inhibitor-Induced Endoplasmic Reticulum (ER) Stress
- Assessment of Mitochondrial Dysfunctions after Sirtuin Inhibition
- Assessing the Effect of Histone Deacetylase Inhibitors on DNA Double-Strand Break Repair by Non-Homologous End Joining
- Assessing SIRT7 Activity In Vivo and In Vitro in Response to DNA Damage
- Methods to Evaluate the Effects of HAT/KAT Inhibition on SIAH2-Driven Reactive Oxygen Species Generation in Helicobacter pylori-Infected Gastric Epithelial Cells