Metagenomics Methods and Protocols
This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct met...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2023, 2023
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| Edition: | 3rd ed. 2023 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Construction of Small-Insert and Large-Insert Metagenomic Libraries
- Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies
- Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds
- Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA
- Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next Generation Sequencing Approach
- Activity-Based Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes
- Functional Metagenomics Approach for the Discovery of Novel Genes Encoding Phosphatase Activity
- Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities
- Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures
- The PET-Degrading Potential of Global Metagenomes: From InSilico Mining to Active Enzymes
- High-Throughput Screening for Thermostable Polyester Hydrolases
- Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System
- Assigning Functions of Unknown Enzymes by High Throughput Enzyme Characterization
- Isolation of a Host-Confined Phage Metagenome Allows the Detection of Phages Both Capable and Incapable of Plaque Formation
- CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis
- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production
- DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms