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Nucleic Acid Aptamers Selection, Characterization, and Application

This updated book reflects improvements in a variety of techniques used to study the aptamer field. Beginning with a section on selection procedures, the volume continues with methods to characterize aptamers’ interaction and structural properties by biophysical approaches, as well as a variety of a...

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Bibliographic Details
Other Authors: Mayer, Günter (Editor), Menger, Marcus M. (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2023, 2023
Edition:2nd ed. 2023
Series:Methods in Molecular Biology
Subjects:
Biophysical Methods
Biophysics
Biology / Technique
Biomaterials
Nucleic Acid
Nucleic Acids
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Automated ssDNA SELEX Using Histidine Tagged Target Proteins
  • Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection
  • Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX
  • Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction
  • RNA Capture-SELEX on Streptavidin Magnetic Beads
  • The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE
  • Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX
  • Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry
  • Determination of Aptamer Structure Using Circular Dichroism Spectroscopy
  • Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis
  • Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance
  • Probing of Fluorogenic RNA Aptamers via SupramolecularFörster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog
  • Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT
  • Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environments
  • Aptasensor for Impedimetric Detection of Lysozyme
  • Biophysical Analysis of Small Molecule Binding to Viral RNA Structures
  • Designing and Expressing Circular RNA Aptamers to Regulate Mammalian Cell Biology
  • Enzyme-Linked OligoNucleotide Assay (ELONA)
  • High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics
  • Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis

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