Fluorescent Proteins Methods and Protocols
This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to th...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2023, 2023
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| Edition: | 1st ed. 2023 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Using FPbase: The Fluorescent Protein Database
- Selecting the Fluorescent Protein for In Vivo Imaging Experiments
- Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo
- Directed Evolution of Fluorescent Proteins in Bacteria
- Fluorescent Proteins: Crystallization, Structural Determination, and Non-Natural Amino Acid Incorporation
- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization
- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization
- CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11 Tag into the Genome of a Human Cell Line
- Fluorescent Protein-Based Approaches for Sub-Cellular Protein Localization in Plants
- Growth Rates Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions
- DNA Visualization Using Fluorescent Proteins
- Quantification ofIntracellular Citrate Concentrations with Genetically Encoded Biosensors
- Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out
- Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae
- Osmium Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells
- Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins
- Determining Protein-Protein Interaction with GFP-Trap Beads