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220928 ||| eng |
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|a 9789264488809
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| 245 |
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|a Test No. 496: In vitro Macromolecular Test Method for Identifying Chemicals Inducing Serious Eye Damage and Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage
|h Elektronische Ressource
|c Organisation for Economic Co-operation and Development
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| 246 |
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|a Essai n° 496 : Méthode d'essai macro-moléculaire in vitro pour l'identification de produits chimiques provoquant des lésions oculaires graves et les produits chimiques ne relevant d'aucune classification pour irritation oculaire ou lésions oculaires graves
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| 260 |
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|a Paris
|b OECD Publishing
|c 2023
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| 300 |
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|a 23 p
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| 653 |
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|a Environment
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| 710 |
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|a Organisation for Economic Co-operation and Development
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|a eng
|2 ISO 639-2
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| 989 |
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|b OECD
|a OECD Books and Papers
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|a OECD Guidelines for the Testing of Chemicals, Section 4
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|a 10.1787/970e5cd9-en
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|a oecd-ilibrary.org
|u https://doi.org/10.1787/970e5cd9-en
|x Verlag
|3 Volltext
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|a 363
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|a The in vitro macromolecular test method is a biochemical in vitro test method that can be used to identify chemicals (substances and mixtures) that have the potential to induce serious eye damage as well as chemicals not requiring classification for eye irritation or serious eye damage. The in vitro macromolecular test method contains a macromolecular reagent composed of a mixture of proteins, glycoproteins, carbohydrates, lipids and low molecular weight components, that when rehydrated forms a complex macromolecular matrix which mimics the highly ordered structure of the transparent cornea. Corneal opacity is described as the most important driver for classification of eye hazard. Test chemicals producing protein denaturation, unfolding and changes in conformation will lead to the disruption and disaggregation of the highly organised macromolecular reagent matrix, and produce turbidity of the macromolecular reagent. Such phenomena is quantified, by measuring the changes in light scattering (at a wavelength of 405 nm using a spectrometer), which is compared to the standard curve established in parallel by measuring the increase in OD produced by a set of calibration substances
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